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conclusive that I am not infected?

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This episode of mistake and foolishness happened on 30th June, 2011. I had an encounter with a Commercial Sex Worker from Bombay. The last few days have been very scary and it looks like I am drifting towards death. Please help me doctor. It has been difficult time all through.

Now, I got my blood sampled on 8th July, 2011 i.e. 8 day PCR RNA test and the results I got on 13th July, 2011 are as under :

Test : Quantification of HIV - 1 RNA by standard procedure on COBAS Taq Man - 48

Method : COBAS Taq Man - 48 with Manual Extraction

Target Selection : Highly Conserved Sequence of Gag gene is selected for amplification and detection

Limit of detection : 47 copies / ml (28.2 IU/ml)

Measuring Range : 47-10,000,000 copies / ml (28.2 IU/ml - 6,000,000 IU/ml)


Please can you comment on the result? Can this be considered reliable and conclusive that I am not infected?

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replied August 9th, 2011
HIV and AIDS Answer A25832
Hello and welcome to e health forum.

The test done was PCR for HIV RNA detection.

The test results are negative for HIV RNA.

I assume that you underwent testing on your own, and were not well counselled.

The PCR -DNA test is a very accurate test for detecting HIV INFECTION. It detects the presence of the HIV DNA, in ‎the blood. Hence its accuracy is 100%. ‎

The PCR-DNA test gives a positive result within 10-14 days after exposure. In your case, the test was conducted at 8 days after exposure, and hence the test result is not valid.

HIV is best diagnosed based on the serological detection in the blood samples.

A person after exposure to HIV can get ‎tested as early as 4 weeks to 12 weeks with the ELISA for HIV antibody test. 90% of normal individuals who have HIV ‎exposure, will seroconvert within 4-6 weeks. The sensitivity of the ELISA test is 99.9%. ‎

You might undergo a HIV PCR -DNA analysis after 2 weeks or a HIV ELISA + p24 combo test after 4 weeks after the exposure. This might give more accurate and valid results.

I hope this helps.

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