Collect the semen in the sterile collection cup or condom by masturbation or intercourse. Be careful not to touch the inside of the cup or condom, and do not scoop any spilled ejaculate back into the container.
Draw back the plunger of the empty syringe, pulling in air, and depress to expel the air. Insert the end of the plunger into the collected semen and draw back on the plunger to pull the semen into the syringe.
Hold the syringe vertically, plunger side down, and tap to release any air bubbles.
Insert the syringe into the vagina as close to the cervix as possible. Do not attempt to insert the syringe into the cervix; this can cause irritation and infection.
Depress the syringe slowly, inserting the sperm into the cervical mucus.
If possible, use clitoral stimulation to have an orgasm. This causes the cervix to draw sperm upward and into the uterus, giving the best chance of fertilization.