Any pregnancy test that is made after you've missed your period is relevant no matter the time of the day. If the test was negative, you are probably not pregnant.

Spotting is not a sign of pregnancy. Spotting can be due to functional (hormonal dysbalance) or organic reasons (infection, polyp, myoma…). Other conditions can also cause lack of menstruation like the presence of a functional ovarian cyst. You can take another pregnancy test to confirm the results. If the test is positive, you are probably pregnant and you should visit your gynecologist. If it is negative you can wait for another 2 months and if the menstrual period still doesn’t come you should visit your gynecologist.