Do a test once a week for 3 weeks, or have a blood test done by a nurse, doctor or clinic.
You do not say, but if you are thinking you can be pregnant you are not on birth control pills (which can cause this breakthrough bleeding)?
If it keeps on happening, you have to see your doctor so that they can make sure there is not something causing it.
Also remember that you should only use a tampon when you flow enough to soak it in the 4 to 8 hour time period. Putting one in when you are drier than that will dry out the area between your vaginal wall and the tampon, and that is not very healthy.
Take care.